Preparation and Application of Monoclonal Antibodies for a Sandwich Enzyme-Linked Immunosorbent Assay of the Major Soybean Allergen, Gly m Bd 30K.
نویسندگان
چکیده
منابع مشابه
Identification of the glycosylation site of a major soybean allergen, Gly m Bd 30K.
A major soybean allergen, Gly m Bd 30K, is a glycoprotein. A peptide containing a sugar chain was isolated from an alpha-chymotrypic hydrolyzate of the allergen. The amino acid sequence analysis of the peptide showed that its sugar chain binds to the Asn170 residue. Furthermore, the sugar moiety of the allergen and peptide was shown to consist of mannose, N-acetylglucosamine, fucose, and xylose...
متن کاملNoteMeasurement of Gly m Bd 30 K , a Major Sandwich Enzyme - linked ImmunosorbentSoybeanAssayAllergen , in Soybean Products
derrnatitis, we found 16 allergenic proteins in the soybean. i) About two-thirds of soybean-sensitive patients examined was shown to have IgE antibodies binding to the 30-kDa protein narned Gly m Bd 30K. This finding suggests that Gly m Bd 30K is a major allergen in the soybean and that it may be a target allergen for hypoallergenization of soybean products. Gly m Bd 30K has been purified to ho...
متن کاملApplication of a modified human enzyme-linked immunosorbent assay kit for diagnosis of hydatidosis in sheep
Cystic hydatid disease (hydatidosis) is one of the most important zoonosis that is caused by the larval stage of Echinococcus granulosus . As its diagnosis by clinical symptoms alone is difficult and confusing, serologic diagnostic techniques are used to confirm the disease. These techniques can also be used for epidemiologic studies. The present study was performed with a commercial human en...
متن کاملA monoclonal antibody-based sandwich enzyme-linked immunosorbent assay for detection of secreted α-defensin.
Paneth cells at the base of small intestinal crypts secrete α-defensins, which contribute to innate immunity and shape composition of enteric microbiota. Efforts to establish a relationship between secreted α-defensins and disease have been hampered by a lack of sensitive assays to quantify luminal α-defensins. Here we report on a highly sensitive sandwich enzyme-linked immunosorbent assay (ELI...
متن کاملIdentification of the soybean allergenic protein, Gly m Bd 30K, with the soybean seed 34-kDa oil-body-associated protein.
The soybean allergenic protein, Gly m Bd 30K [Ogawa et al., J. Nutr. Sci. Vitaminol., 37, 555-565 (1991)] which is most strongly and frequently recognized by the IgE antibodies in sera of soybean-sensitive patients with atopic dermatitis, has been characterized. The allergen was isolated from the crude 7S-globulin fraction as an oligomeric form with a molecular weight of more than 3000,000 by g...
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ژورنال
عنوان ژورنال: Journal of Nutritional Science and Vitaminology
سال: 1993
ISSN: 0301-4800,1881-7742
DOI: 10.3177/jnsv.39.389